MEASUREMENT OF PERMEATING LEVELS OF CRYOPROTECTANT DURING OVARIAN TISSUE CRYOPRESERVATION USING H-1-NMR SPECTROSCOPY IN HUMAN AND PORCINE OVARIES

The concentrations of dimethyl sulphoxide in ovarian tissues of porcin e and human origin, during a standard cryopreservation procedure, were measured by H-1 NMR spectroscopy. Results suggested that tissue loadi ng and elution of the cryoprotectant were similar in tissues from both sources. The final concentrations of dimethyl sulphoxide achieved usi ng the protocol were only approximately 50% of the theoretical maximum if full equilibration with tissue water had taken place. The results are discussed in relation to the response of ovarian tissue to cryopre servation.