Search for molecular markers linked to Liriomyza trifolii resistance in tomato

Resistance to many arthropods, including Liriomyza species, is known to be present in accessions of Lycopersicon hirsutum (f. typicum or f. glabratum) . From the cross L. esculentum cv. Moneymaker and L. hirsutum f. glabratum G1561 100 F-2 plants were screened in a no-choice test for resistance to Li riomyza trifolii. The Bulked Segregant Analysis approach was used to find R andom Amplified Polymorphic DNA markers linked to resistance. Two markers w ere located on chromosome 2. Restriction Fragment Length Polymorphisms cons tructed a more detailed genetic linkage map for part of chromosome 2. Krusk al-Wallis analysis showed that this chromosome harbored a Quantitative Trai t Locus (QTL) for number of pupae, number of mines and damage. At least one major QTL is essential for resistance and this QTL is located on chromosom e 2 nearby the location of the tomato probe TG451.