The frequency of the GSTM1 gene in patients with nonpolyposis colorectal ca
ncer (CRC) (n = 70) and in subjects with colonic polyps (n = 27) was evalua
ted and compared with healthy individuals (n = 145).
Patients with CRC were divided into the three groups: patients coming from
the families with hereditary nonpolyposis colorectal cancer (HNPCC) (n = 17
); patients with a high risk of HNPCC who were referred to as suspected of
HNPCC (n = 25); patients with sporadic colorectal cancer without clinical f
eatures of hereditary tumours (n = 28).
A simple polymerase chain reaction (PCR) - based assay to identify GSTM1 nu
lled and positive (non-nulled) genotype was used.
No significant differences in frequency of nulled individuals were observed
in both patients with HNPCC and patients suspected of HNPCC as well as in
subjects with colonic polyps. The most interesting observation was made in
the group of patients with sporadic CRC. Twenty individuals (71.4 %) of the
group were GSTM1 deficient which was significantly different from the cont
rol population (p < 0.04). The above data indicate that the absence of the
GSTM1 gene is associated with a greater risk of sporadic colorectal cancer.
There is an increase in the overall risk of similar to 2.5. as compared wi
th the control population.