Immunolocalization of cyclooxygenase-2 in the macula densa of human elderly

To gain insight into the role of prostanoids in human kidney function, we e xamined the distribution of cyclooxygenase (COX) 1 and COX-2 by immunofluor escence and immunohistochemistry in human kidneys from adults of various ag e groups. COX-1 was detected in the collecting ducts, thin loops of Henle a nd portions of the renal vasculature, COX-2 was detected in the renal vascu lature, medullary interstitial cells, and the macula densa, In addition, CO X-2 immunoreactivity was noted in afferent arteries and the macula densa of the renal cortex and was more evident in the kidneys of older adults. (C) 1999 Federation of European Biochemical Societies.