The hemagglutinating activities of Pseudomonas aeruginosa lectins PA-IL and PA-IIL exhibit opposite temperature profiles due to different receptor types
N. Gilboa-garber et D. Sudakevitz, The hemagglutinating activities of Pseudomonas aeruginosa lectins PA-IL and PA-IIL exhibit opposite temperature profiles due to different receptor types, FEMS IM MED, 25(4), 1999, pp. 365-369
The two Pseudomonas aeruginosa lectins PA-IL and PA-IIL, which are very sim
ilar in subunit size, composition and properties, but differ in carbohydrat
e specificity, were shown to exhibit opposite temperature profiles in hemag
glutination tests. The galactophilic PA-IL, which interacts with the erythr
ocyte I antigen (together with B or P system antigens), resembles Ii system
-specific 'cold hemagglutinins' (including antibodies and lectins of animal
s and plants) in low (4 degrees C) temperature optimum, while the hemagglut
ination by the fucose- and mannose-binding PA-IIL (like that of antibodies
and lectins which do not bind to these antigens) increases on raising the t
emperature from 4 to 37 degrees C and even to 42 degrees C. The preferentia
l production of both P. aeruginosa lectins at 28 degrees C and their much s
tronger interaction with enzyme (protease or sialidase)-damaged cells, as w
ell as the lower temperature optimum (4 degrees C) of PA-IL-binding to the
host cells, may be associated with the saprophytic rather than parasitic de
signation of this bacterium. (C) 1999 Federation of European Microbiologica
l Societies. Published by Elsevier Science B.V. All rights reserved.