A direct chemiluminescence method for the determination of nucleic acids using Ru(phen)(3)(2+)-Ce(IV) system

A direct chemiluminescence method for the determination of nucleic acids ha s been developed based on the enhancement of nucleic acids on the chemilumi nescence light emission of the reaction between Ru(phen)(3)(2+)(phen=1,10-p henanthroline) and Ce(IV). Under the optimum conditions, the calibration gr aphs are linear in the range of 5.0 x 10(-8)-5.0 x 10(-5) g/mL for calf thy mus DNA, 8.0 x 10(-8)-5.0 x 10(-5) g/mL for fish sperm DNA and 1.0 x 10(-7) -3.0 x 10(-5) g/mL for yeast RNA, respectively. The limits of detection are 1.0 x 10(-5) g/mL for calf thymus DNA, 3.1 x 10(-8) g/mL for fish sperm DN A and 5.2 x 10(-8) g/mL for yeast RNA, respectively. The final procedure al lows the successful determination of calf thymus DNA, fish sperm DNA and ye ast RNA in six synthetic samples. This method is simple, rapid and specific .