SUBSTRATE (GELATIN) GEL-ELECTROPHORETIC METHOD FOR ANALYSIS OF PROTEASE ACTIVITY IN INSECT (SF-9) CELLS

Authors
NAGGIE S HU YC PULLIAMHOLOMAN TR BENTLEY WE
Citation
S. Naggie et al., SUBSTRATE (GELATIN) GEL-ELECTROPHORETIC METHOD FOR ANALYSIS OF PROTEASE ACTIVITY IN INSECT (SF-9) CELLS, Biotechnology techniques, 11(5), 1997, pp. 297-300
Citations number
14
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
5
Year of publication
1997
Pages
297 - 300
Database
ISI
SICI code
0951-208X(1997)11:5<297:S(GMFA>2.0.ZU;2-8
Abstract
A method for detection, quantification, and characterization of protea se activities present in the insect cell/baculovirus protein expressio n system was demonstrated. Proteases were found of 39, 44, and 52 kDa molecular weight. Enzymatic inhibitors (antipain, chymostatin, EDTA, p epstatin A), cofactors (e.g., ATP, Mg2+, Ca2+), pH, and substrates (ge latin, beta-galactosidase, bovine serum albumin) were varied to improv e and characterize the activities.