OPTIMIZED PRODUCTION OF THE SOLUBLE HUMAN INTERFERON-ALPHA RECEPTOR (IFNAR) EXPRESSED IN ESCHERICHIA-COLI

Authors
HIRATA RDC HIRATA MH LEVY DE NGUYEN NY
Citation
Rdc. Hirata et al., OPTIMIZED PRODUCTION OF THE SOLUBLE HUMAN INTERFERON-ALPHA RECEPTOR (IFNAR) EXPRESSED IN ESCHERICHIA-COLI, Biotechnology techniques, 11(5), 1997, pp. 301-305
Citations number
15
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
5
Year of publication
1997
Pages
301 - 305
Database
ISI
SICI code
0951-208X(1997)11:5<301:OPOTSH>2.0.ZU;2-E
Abstract
An optimized procedure has been developed for production and purificat ion of the human interferon or receptor (IFNAR) expressed in E. coli a s fusion protein with glutathione S-transferase (GST). Expression indu ced at 30 degrees C and cell disruption performed at pH = 9.0 in prese nce of detergents reduced the inclusion body formation and generated u p to 20% of the fusion protein in the soluble form. The recombinant IF NAR, recovered in soluble and renaturated forms, was able to block the antiviral and antiproliferative activities of IFN alpha B.