DEVELOPMENT OF CONSTITUTIVE DEXTRANSUCRASE HYPER-PRODUCING MUTANTS OFLEUCONOSTOC-MESENTEROIDES USING THE SYNCHROTRON-RADIATION IN THE 70-1000 EV REGION

Authors
KIM D KIM DW LEE JH PARK KH DAY LM DAY DF
Citation
D. Kim et al., DEVELOPMENT OF CONSTITUTIVE DEXTRANSUCRASE HYPER-PRODUCING MUTANTS OFLEUCONOSTOC-MESENTEROIDES USING THE SYNCHROTRON-RADIATION IN THE 70-1000 EV REGION, Biotechnology techniques, 11(5), 1997, pp. 319-321
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
5
Year of publication
1997
Pages
319 - 321
Database
ISI
SICI code
0951-208X(1997)11:5<319:DOCDHM>2.0.ZU;2-#
Abstract
After irradiation with photons in the energy range of 70-1000 eV using the synchrotron radiation facility at Pohang, Korea, dextransucrase c onstitutive and hyper-producing mutants from Leuconostoc mesenteroides were isolated. The mutant (B-512FMCM) produced 13 times higher activi ty and showed complete constitutivity for dextransucrase production. I t synthesized the same dextran as B-512FMC. The dextransucrase of the mutant transferred glucose from dextran to maltose. This novel method is a new technique for the development of industrial microorganisms.