Clinical efficacy of GnRH analogue (buserelin) and oestradiol benzoate treatments in anoestrous buffaloes

1he clinical efficacy of GnRH analogue (buserlin) and oestradiol benzoate t reatments in advancing the breeding seasons in anoestrous buffaloes was eva luated. Anoestrous buffaloes (31) were divided into 3 groups according to t he treatment given to them. Buserelin (group 1, n = 10) and oestradiol (gro up 2, n = 13) were administered in lower(10 mu g and 1 mg, respectively) or higher doses (20 mu g and 2 mg, respectively) during the period extending from July to October, untreated anoestrous buffaloes (control 1, n = 8) and regularly cycling buffaloes (control 2, n = 9) were kept as controls. Buse relin included ovulatory heat within 20-32 days in 9 out of 13 treated buff aloes and 5 of them became pregnant. Oestradiol was able to induce ovulator y heat within 20-32 days in 9 out of 13 treated buffaloes, and 4 out of 7 b uffaloes capable of conceiving became pregnant in this group. Buffaloes (2) in the control group expressed oestrous within 11.00+/-4.34 days and only 1 of them conceived. Higher doses of buserelin (20 mu g) and oestradiole (2 mg) were effective in inducing cyclicity. It was concluded that both buser elin and oestradiol benzoate, especially in higher doses (20 mu g and 2 mg, respectively) were highly effective in including oestrus in anoestrous buf faloes during the transition period from low-to peak-breeding season.