Identification and characterisation of two allergens from the dust mite Acarus siro, homologous with fatty acid-binding proteins

Citation
Tlj. Eriksson et al., Identification and characterisation of two allergens from the dust mite Acarus siro, homologous with fatty acid-binding proteins, INT A AL IM, 119(4), 1999, pp. 275-281
Citations number
35
Categorie Soggetti
Immunology
Journal title
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
ISSN journal
10182438 → ACNP
Volume
119
Issue
4
Year of publication
1999
Pages
275 - 281
Database
ISI
SICI code
1018-2438(199908)119:4<275:IACOTA>2.0.ZU;2-G
Abstract
Background: Dust mites are a major cause of allergic disease worldwide. The dust mite Acarus sire is an inducer of occupational allergy among farmers, but sensitisation has also been found in non-farming populations. Methods: A degenerate primer was designed to the N-terminal amino acid sequence of a 15-kD IgE-binding protein in A. siro extract. The cDNA sequence was obtai ned by using reverse transcriptase polymerase chain reaction, standard clon ing and sequencing techniques. The protein was expressed in Escherichia col i with a 6-histidine tag at its C-terminus. Immunoblotting of the recombina nt protein and whole extract was performed using patient sera. Results and conclusion: 15 and 17-kD allergens were identified in a fraction of A, sire extract. The cDNA of the 15-kD allergen was isolated, cloned and sequenced and the allergen was expressed as a recombinant protein. The calculated mo lecular weight of the cDNA-encoded protein is 14.2 kD. The predicted amino acid sequence has one potential N-glycosylation site at position 4-6 and a cytosolic fatty acid-binding protein signature at position 5-22. The protei n has 64% sequence identity with Blo t 13, an allergen from the dust mite B lomia tropicalis, as well as homology with several other fatty acid-binding proteins (FABPs) from different organisms. The allergen was named Aca s 13 and was recognised strongly by 3 of 13 (23%) of the subjects investigated. The amino acid sequence of the 17-kD protein was partly determined and it also showed high sequence homology with Blo t 13 and FABPs.