Antiviral activity of enterocin CRL35 against herpesviruses

Citation
Mb. Wachsman et al., Antiviral activity of enterocin CRL35 against herpesviruses, INT J ANT A, 12(4), 1999, pp. 293-299
Citations number
21
Categorie Soggetti
Microbiology
Journal title
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
ISSN journal
09248579 → ACNP
Volume
12
Issue
4
Year of publication
1999
Pages
293 - 299
Database
ISI
SICI code
0924-8579(199908)12:4<293:AAOECA>2.0.ZU;2-L
Abstract
Enterocin CRL35 is an antibacterial polypeptide of 3.5 x 10(3) Da produced by Enterococcus faecium CRL35. A series of experiments are described that s how the enterocin also had antiviral activity against thymidine-kinase posi tive (tk(+)) and deficient (tk(-)) strains of herpes simplex (HSV) type 1 a nd 2 in Vero and BHK-21 cells. This activity was observed at 100 mu g/ml, 1 5-fold lower than the cytotoxic concentration. In both cell lines there was a 2 log inhibition of infectivity. The compound inhibited viral multiplica tion in a dose-dependent manner and had no virucidal effect. Enterocin CRL3 5 also inhibited the virion-associated host shutoff in infected Vero cells showing that intracellular viral multiplication was affected. (C) 1999 Else vier Science B.V. and International Society of Chemotherapy. All rights res erved.