Cdc42 and Rac1 regulate the interaction of IQGAP1 with beta-catenin

IQGAP1, a target of Cdc42 and Bad small GTPases, directly interacts with be ta-catenin and negatively regulates E-cadherin-mediated cell-cell adhesion by dissociating alpha-catenin from the cadherin-catenin complex in vivo (Ku roda, S., Fukata, M., Nakagawa, M., Fujii, R., Nakamura, T., Ookubo, T., Iz awa, I., Nagase, T., Nomura, N., Tani, H., Shoji, I,, Matsuura, Y., Yonehar a, S,, and Kaibuchi, IT. (1998) Science 281, 832-855). Here we investigated how Cdc42 and Rad regulate the IQGAP1 function. IQGAP1 interacted with the amino-terminal region (amino acids 1-183) of beta-catenin, which contains the alpha-catenin-binding domain. IQGAP1 dissociated alpha-catenin from the beta-catenin-alpha-catenin complex in a dose-dependent manner in vitro. Gu anosine 5'-(3-O-thio)triphosphate (GTP gamma S) glutathione S-transferase ( GST)Cdc42 and GTP gamma S.GST-Rac1 inhibited the binding of IQGAP1 to beta- catenin in a dose-dependent manner vitro, whereas neither GDP.GST-Cdc42, GD P.GST-Rac1, in nor GTP gamma S GST-RhoA did. The coexpression of dominant a ctive Cdc42 with IQGAP1 suppressed the dissociation of alpha-catenin from t he cadherin-catenin complex induced by the overexpression of IQGAP1 in L ce lls expressing E-cadherin (EL cells). Consistent with this, the overexpress ion of either dominant negative Cdc42 or Rad resulted in the reduction of E -cadherin-mediated cell adhesive activity in EL cells. These results indica te that Cdc42 and Rad negatively regulate the IQGAP1 function by inhibiting the interaction of IQGAP1 with beta-catenin, leading to stabilization of t he cadherin-catenin complex.