Detection and identification of sulphonamide drugs in municipal waste water by liquid chromatography coupled with electrospray ionisation tandem massspectrometry

High-performance liquid chromatography coupled with positive-ion electrospr ay ionisation tandem mass spectrometry was used for the determination and c onfirmation of 13 sulphonamide drugs in environmental water samples in the low ng/L-range. Enrichment with concentration factors of 130-670 was perfor med by solid phase extraction, achieving recoveries of 50 to 90%. After gra dient elution HPLC, detection and quantification was performed using select ed reaction monitoring (SRM) with limits of detection between 0.2 and 3.7 m u g/L. Confirmation was obtained by either SRM transitions of collision ind uced dissociation reactions or daughter ion mass spectra. Primary and secon dary effluents of municipal waste water treatment plants and different surf ace waters were examined. The compounds sulphamethoxazole and sulphadiazine were detected and confirmed with concentrations ranging between 30-2000 ng /L and 10-100 ng/L, respectively. The compound sulphamethizole was detected in low amounts but could not be positively confirmed. (C) 1999 Elsevier Sc ience B.V. All rights reserved.