High-performance liquid chromatographic assay of formycin A in plasma after solid-phase extraction

A new, simple and accurate high-performance liquid chromatography (HPLC) me thod for the determination of formycin A in plasma is presented. The sample s were chromatographed on a LiChrosorb RP-18 column after purification usin g a Bakerbond SPE column. The mobile phase was methanol-0.067 M phosphate b uffer, pH 4.20 (1:4, v/v) containing 0.005 M sodium hexanesulfonate. Azathi oprine was applied as an internal standard. UV detection was carried out at 293 nm. The method was tested for linearity (over the range 0.1-9.0 mu g/m l). The recovery was 91.89% (mean). The described method has been successfu lly applied to the quantitative determination of formycin A in plasma and s hould be useful for clinical and bioavailability investigations. (C) 1999 E lsevier Science B.V. All rights reserved.