Separation of bovine serum albumin and its monoclonal antibody from their immunocomplexes by sodium dodecyl sulfate-capillary gel electrophoresis andits application in capillary electrophoresis-based immunoassay

A non-competitive immunoassay was performed by sodium dodecyl sulfate-capil lary gel electrophoresis with UV detection using bovine serum albumin (BSA) and monoclonal anti-BSA. BSA, anti-BSA and their immunocomplexes were well resolved under non-denaturing conditions. A linear calibration curve was o btained and can be used for the quantification of anti-BSA. The limit of de tection of anti-BSA was 0.1 mu M under the present conditions. Compared wit h capillary zone electrophoresis, we believed that this method has the pote ntial to be used as a more general format for performing capillary electrop horesis-based immunoassay of medium- and large-sized analytes. (C) 1999 Els evier Science B.V. All rights reserved.