FHIT GENE-EXPRESSION IN HUMAN OVARIAN, ENDOMETRIAL, AND CERVICAL-CANCER CELL-LINES

Citation
Dt. Hendricks et al., FHIT GENE-EXPRESSION IN HUMAN OVARIAN, ENDOMETRIAL, AND CERVICAL-CANCER CELL-LINES, Cancer research, 57(11), 1997, pp. 2112-2115
Citations number
15
Categorie Soggetti
Oncology
Journal title
ISSN journal
00085472
Volume
57
Issue
11
Year of publication
1997
Pages
2112 - 2115
Database
ISI
SICI code
0008-5472(1997)57:11<2112:FGIHOE>2.0.ZU;2-#
Abstract
(1)The fragile histidine triad (FHIT) gene, located at 3p14.2, has bee n shown to be altered in numerous epithelial cancers, Because previous studies have shown a loss of heterozygosity and cytogenetic abnormali ties at the 3p region in ovarian, endometrial, and cervical carcinomas , we examined the status of the FHIT gene in 14 ovarian, 8 cervical, a nd 4 endometrial human cancer cell lines, RNA was isolated and subject ed to reverse transcription-PCR to amplify the FHIT gene transcript, S ixty-three % (5 of 8) of cervical cell lines, 14% (2 of 14) of ovarian cell lines, and none (0 of 4) of the endometrial cell lines displayed aberrantly migrating FHIT transcripts. DNA sequencing demonstrated th at the aberrantly migrating bands primarily lacked exons 5, 6, and 7 ( with other exon losses also observed), resulting in shorter mRNA trans cripts, Southern blot analysis of DNA from five of the cervical carcin omas demonstrated alterations in four of them, three of which had exhi bited no normally sized FHIT transcripts, The results suggest that the expression of the FHIT gene may be altered, in cervical tumor tissue, potentially implicating this gene in cervical tumorigenesis, whereas the involvement of this gene appears to be less important in the devel opment of ovarian and endometrial cancer.