Photochemical inactivation of recombinant baculovirus

A method for the inactivation of aqueous suspensions of recombinant baculov iruses was developed using the combined effects of 4,5,8, trimethyl psorale n (trioxalen) and long-wave length UV illumination. A series of dose-effect titrations established the optimum conditions for the complete inactivatio n of a recombinant baculovirus expressing the glycoprotein D gene of Aujesz ky's disease virus. The method was found to be equally effective against pa rental baculoviruses. The photochemical inactivation method proved simple, rapid and effective with minimal loss of biological activity in a recombina nt protein subsequently used in an ELISA. Crown copyright (C) 1999 Elsevier Science B.V. All rights reserved.