The coupled bioluminescent enzyme system luciferase-NADH:FMN-oxidoreductase
was used as a biotest in ecological monitoring of the health resort salt l
ake Shira (South Siberia, Russia). The technique was adapted to saltwater c
onditions. Bioluminescence kinetic parameters sensitive to pollutants were
determined. Conditions for the use of bacterial bioluminescence biotests in
salty environmental media were established. Copyright (C) 1999 John Wiley
& Sons, Ltd.