A. Pettersson et al., DETERMINATION OF DIMETHYLATED ARGININES IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical sciences and applications, 692(2), 1997, pp. 257-262
Citations number
22
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Using high-performance liquid chromatography (HPLC) with multigradient
elution, NG,NG-dimethyl-L-arginine (asymmetric-DMA ADMA) and N-G,N'(G
)-dimethyl-L-arginine (symmetric-DMA, SDMA) can be separated from huma
n plasma samples. The dimethylarginine compounds in plasma, after extr
action with a cation-exchange column, are converted to fluorescent der
ivatives with o-phthaldialdehyde (OPA) in an alkaline medium and the d
erivatives are separated simultaneously within 50 min on a reversed-ph
ase column (Ultracarb 3 ODS(20)). The recoveries of ADMA and SDMA are
over 80% and the method permits quantitative determination of dimethyl
ated arginines at concentrations as low as 0.1 mu mol/1 in human plasm
a.