Dc. Hankins et Ed. Kharasch, DETERMINATION OF THE HALOTHANE METABOLITES TRIFLUOROACETIC-ACID AND BROMIDE IN PLASMA AND URINE BY ION CHROMATOGRAPHY, Journal of chromatography B. Biomedical sciences and applications, 692(2), 1997, pp. 413-418
Citations number
12
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Halothane (CF3CHClBr), a widely used volatile anesthetic, undergoes ex
tensive biotransformation in humans. Oxidative halothane metabolism yi
elds the stable metabolites trifluoroacetic acid and bromide which can
be detected in plasma and urine. To date, analytical methodologies ha
ve either required extensive sample preparation, or two separate analy
tical procedures to determine plasma and urine concentrations of these
analytes. A rapid and sensitive method utilizing high-performance liq
uid chromatography-ion chromatography (HPLC-IC) with suppressed conduc
tivity detection was developed for the simultaneous detection of both
trifluoroacetic acid and bromide in plasma and urine. Sample preparati
on required only ultrafiltration. Standard curves were linear (r(2) gr
eater than or equal to 0.99) from 10 to 250 mu M trifluoroacetic acid
and 2 to 5000 mu M bromide in plasma and 10 to 250 mu M trifluoroaceti
c acid and 2 to 50 mu M bromide in urine. The assay was applied to qua
ntification of trifluoroacetic acid and bromide in plasma and urine of
a patient undergoing halothane anesthesia.