Immunohistochemical analysis of HLA class II antigens and tumor infiltrating mononuclear cells in renal cell carcinoma: Correlation with clinical andhistopathological data
D. Brasanac et al., Immunohistochemical analysis of HLA class II antigens and tumor infiltrating mononuclear cells in renal cell carcinoma: Correlation with clinical andhistopathological data, NEOPLASMA, 46(3), 1999, pp. 173-178
Cryostat sections of 37 renal cell carcinomas (RCC) - 25 clear cell type, 1
0 granular and 2 chromophobe - were studied with indirect immunoperoxidase
method applying monoclonal antibodies (MoAb) to HLA-DR, -DP and -DQ antigen
s for analysis of HLA class II antigens, and anti-CD14, -CD3, -CD4 and -CD8
MoAb for tumor infiltrating mononuclear cells (TIM). Number of positive ce
lls was estimated semiquantitatively and results of immunohistochemical inv
estigation were correlated with clinical (patient age and sex, tumor size a
nd TNM stage) and histopathological (cytology, histology, grade) characteri
stics of RCC. All RCC expressed HLA-DR, 92% -DQ and 73% -DP antigens with l
evel of expression in hierarchy DR>-DQ>-DP, but no statistically important
correlation could be established with any of the histopathological or clini
cal parameters analyzed. Monocytes were more abundant than T lymphocytes an
d CD4+ than CD8+ T cells, whereas tumors with T lymphocyte predominance and
approximately equal number of CD4+ and CD8+ T cells had greatest average d
iameter. Inadequate activation of T lymphocytes by tumor cells (despite cap
ability of antigen presentation) could be the reason for association of par
ameters which indicates more aggressive tumor behavior with aberrant HLA cl
ass II antigen expression on RCC.