CHROMOSOME ASSIGNMENT OF 115 EXPRESSED SEQUENCE TAGS (ESTS) FROM HUMAN SKELETAL-MUSCLE

The chromosome assignment of 115 expressed sequence tags (ESTs) from h uman skeletal muscle, 101 of which identify unknown human genes, is re ported. The ESTs were selected among over 4,000 obtained from systemat ic sequencing of a skeletal muscle cDNA library containing 3' portions of the mRNAs. Chromosome assignments were obtained by PCR amplificati on of two panels of human x rodent somatic cell hybrids. Analysis of t hese preliminary data suggests a nonrandom distribution of muscle ESTs in the human chromosome complement. The unexpected occurrence of mult iple chromosome localizations for some ESTs is discussed.