GENERATION OF SMALL INSERT GENOMIC FISH PROBES WITH HIGH SIGNAL INTENSITY SUITABLE FOR DELETION MAPPING

Authors
DREYLING MH OLOPADE OI BOHLANDER SK
Citation
Mh. Dreyling et al., GENERATION OF SMALL INSERT GENOMIC FISH PROBES WITH HIGH SIGNAL INTENSITY SUITABLE FOR DELETION MAPPING, Cytogenetics and cell genetics, 76(3-4), 1997, pp. 202-205
Citations number
12
Categorie Soggetti
Cell Biology","Genetics & Heredity
Journal title
Cytogenetics and cell genetics → ACNP
ISSN journal
03010171
Volume
76
Issue
3-4
Year of publication
1997
Pages
202 - 205
Database
ISI
SICI code
0301-0171(1997)76:3-4<202:GOSIGF>2.0.ZU;2-2
Abstract
We have developed a method to generate FISH probes from small (2-4 kb) nonrepetitive genomic restriction fragments. The probes showed a high hybridization efficiency of up to 90 % in metaphase cells from normal peripheral blood. With the use of these probes, homozygous as well as hemizygous 9p deletions were reliably identified in nine leukemia-der ived cell lines.