Tomato mosaic tobamovirus is a very stable plant virus with a wide host ran
ge, which has been detected in plants, soil, water, and clouds. Because of
its stability and prevalence in the environment, we hypothesized that it mi
ght be preserved in ancient ice. We detected tomato mosaic tobamovirus RNA
by reverse-transcription polymerase chain reaction amplification in glacial
ice subcores <500 to approximately 140,000 years old from drill sites in G
reenland. Subcores that contained multiple tomato mosaic tobamovirus genoty
pes suggest diverse atmospheric origins of the virus, whereas those contain
ing tomato mosaic tobamovirus sequences nearly identical to contemporary on
es suggest that recent tomato mosaic tobamovirus populations have an extend
ed age structure. Detection of tomato mosaic tobamovirus in ice raises the
possibilities that stable viruses of humans and other hosts might be preser
ved there, and that entrapped ancient Viable viruses may be continually or
intermittently released into the modern environment.