Altered redox affinity of xanthine oxidase active sites by copper(II) ions

Authors
Mondal, MS Mitra, S
Citation
Ms. Mondal et S. Mitra, Altered redox affinity of xanthine oxidase active sites by copper(II) ions, P I A S-CH, 111(3), 1999, pp. 501-508
Citations number
31
Categorie Soggetti
Chemistry
Journal title
PROCEEDINGS OF THE INDIAN ACADEMY OF SCIENCES-CHEMICAL SCIENCES
ISSN journal
02534134 → ACNP
Volume
111
Issue
3
Year of publication
1999
Pages
501 - 508
Database
ISI
SICI code
0253-4134(199906)111:3<501:ARAOXO>2.0.ZU;2-C
Abstract
The interaction of Cu2+ ion with the redox centres of xanthine oxidase (XO) has been investigated using optical difference spectroscopic measurements. Anaerobic enzyme-reduction experiments using controlled and excess substra tes (xanthine and NADH) have been performed to investigate the perturbation of XO active sites in the presence of Cu2+ ions. The results indicate an o verall alteration in the redox affinities (i.e. affinity to accept electron s) of the active sires of XO by Cu2+ ion.