Compactness of the denatured state of a fast-folding protein measured by submillisecond small-angle x-ray scattering

Authors
Pollack, L Tate, MW Darnton, NC Knight, JB Gruner, SM Eaton, WA Austin, RH
Citation
L. Pollack et al., Compactness of the denatured state of a fast-folding protein measured by submillisecond small-angle x-ray scattering, P NAS US, 96(18), 1999, pp. 10115-10117
Citations number
30
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN journal
00278424 → ACNP
Volume
96
Issue
18
Year of publication
1999
Pages
10115 - 10117
Database
ISI
SICI code
0027-8424(19990831)96:18<10115:COTDSO>2.0.ZU;2-M
Abstract
Time-resolved small-angle x-ray scattering was used to measure the radius o f gyration of cytochrome c after initiation of folding by a pH jump. Submil lisecond time resolution was obtained with a microfabricated diffusional mi xer and synchrotron radiation. The results show that the protein first coll apses to compact denatured structures before folding very fast to the nativ e state.