Volatile anesthetics block actin-based motility in dendritic spines

Dendritic spines form the postsynaptic contact sites for most excitatory sy napses in the brain. Spines occur in a wide range of different shapes that can vary depending on an animal's experience or behavioral status. Recently we showed that spines on living neurons can change shape within seconds in a process that depends on actin polymerization. We have now found that thi s morphological plasticity is blocked by inhalational anesthetics at concen trations at which they are clinically effective. These volatile compounds a lso block actin-based motility in fibroblasts, indicating that their action is independent of neuron-specific components and thus identifying the acti n cytoskeleton as a general cellular target of anesthetic action. These obs ervations imply that inhibition of actin dynamics at brain synapses occurs during general anesthesia and that inhalational anesthetics are capable of influencing the morphological plasticity of excitatory synapses in the brai n.