Kinetics and pathways of oxidation of o-phenylenediamine, 3,3 '-dimethoxybenzidine, and 3,3 ',5,5 '-tetramethylbenzidine with hydrogen peroxide, catalyzed with horse radish peroxidase immobilized on various supports

Products of oxidation of o-phenylenediamine, 3,3'-dimethoxybenzidine, and 3 ,3',5,5'-tetramethylbenzidine with hydrogen peroxide, catalyzed with peroxi dase from horse radish roots (native and immobilized on chromatographic pap er and polyurethane foam) were studied in comparison by absorption and diff use reflection spectroscopy. The kinetics and pathways of the enzyme-cataly zed reactions differ depending on whether the reaction was performed in sol ution with native peroxidase or on the above-mentioned supports with the im mobilized enzyme.