Characterisation of cells in regenerating cartilage from autotransplanted perichondrium. immunohistochemical expression of smooth-muscle actin, desmin, vimentin, and Ki-67
A. Ljung et al., Characterisation of cells in regenerating cartilage from autotransplanted perichondrium. immunohistochemical expression of smooth-muscle actin, desmin, vimentin, and Ki-67, SC J PLAST, 33(3), 1999, pp. 257-266
Citations number
30
Categorie Soggetti
Surgery
Journal title
SCANDINAVIAN JOURNAL OF PLASTIC AND RECONSTRUCTIVE SURGERY AND HAND SURGERY
Autotransplanted perichondrium from rib and ear sutured to the knee joints
of 26 rabbits has been examined with immunohistochemistry and shows certain
structural, functional, and proliferative characteristics of regenerating
cartilage. Cryostat sections have been examined for the expression of smoot
h-muscle actin (SMA), desmin, vimentin, and Ki-67. In this rabbit model of
perichondrial grafting SMA staining showed vivid vessel regeneration, parti
cularly in the proliferating stage about two to three weeks after grafting,
and no vessels in more mature parts one month or more after transplantatio
n. Desmin staining showed expression and distribution similar to SMA. Vimen
tin staining shows the cytoskeleton of regenerating cartilaginous tissue an
d makes cellular borders apparent. Immunohistochemical expression of Ki-67
is constantly negative in perichondrial tissue from rib and ear before tran
splantation, clearly positive in the proliferative stage, but there is no e
xpression in maturing cartilage. The study also shows that all human antibo
dies used are applicable in a rabbit model.