Quantum leaps in scientific understanding often result from new investigati
ve techniques. For example, the use of nucleic acids as biomarkers has led
to a mini-revolution in microbial ecology, allowing microorganisms to be st
udied in their natural environment without cultivation, a procedure that of
ten yielded a small, unrepresentative fraction of the community. In this ov
erview, we discuss how nucleic acid based methods, together with new micros
copic techniques and more traditional approaches, could be used to elucidat
e the roles played by microorganisms in encouraging the precipitation of ca
rbonate minerals. (C) 1999 Elsevier Science B.V. All rights reserved.