Identification of a new lepidopteran sex pheromone in picogram quantities using an antennal biodetector: (8E,10Z)-tetradeca-8,10-dienal from Cameraria ohridella

A major sex attractant released by the virgin female of the horse-chestnut leafminer Cameraria ohridella Deschka et Dimic (Lepidoptera: Gracillariidae ) which devastates horse-chestnut trees in Europe, was identified in picogr am quantities as (8E,10Z)-tetradeca-8,10-dienal without using spectral meth ods. The identification solely relied on gas chromatography with electroant ennographic detection (GC-EAD), calculation of Kovats' indices of the activ e principle on different GC phases, and construction of antennal response s pectra (EAG response profiles) to C-12 and C-14 saturated and unsaturated s tandards with different functional groups. The dienal was prepared by a ste reospecific synthesis and shown to be highly active for conspecific males i n pg amounts and Fully comparable to the natural substance. (C) 1999 Elsevi er Science Ltd. All rights reserved.