Yawning is a phylogenetically old, stereotyped event that occurs alone or a
ssociated with stretching and/or penile erection in humans, in animals from
reptiles to birds and mammals, under different conditions. Several neurotr
ansmitters and neuropeptides are involved in its control at the central lev
el. One of these at the level of the paraventricular hypothalamic nucleus (
PVHN) is nitric oxide (NO). First, NO synthase inhibitors injected into thi
s hypothalamic nucleus prevent yawning induced by dopamine agonists, oxytoc
in or N-methyl-D-aspartic acid (NMDA), which induce yawning by activating P
VHN oxytocinergic neurons projecting to extrahypothalamic brain areas. The
inhibitory effect of NO synthase inhibitors was not observed when these com
pounds were given concomitantly with L-arginine, the precursor of NO. Secon
d, dopamine agonists, NMDA and oxytocin given at doses that induce yawning,
increase NO production in the PVHN, as determined by in vivo microdialysis
. Conversely, the opiate morphine, which prevents yawning induced by dopami
ne agonists, oxytocin and NMDA, also prevents the increase in the paraventr
icular NO production induced by these compounds. Third, NO donors, such as
nitroglycerin, sodium nitroprusside and hydroxylamine, induce yawning when
injected into the PVHN apparently by activating oxytocinergic transmission.
Since guanylate cyclase inhibitors and NO scavengers (hemoglobin) injected
into the PVHN do not prevent drug-induced yawning, nor 8-Br-cGMP injected
into the PVHN induces this behavioral response, it is likely that NO acts a
s an intracellular rather than an intercellular modulator inside the PVHN o
xytocinergic neurons in which NO is formed to facilitate the expression of
this phylogenetically old event by guanylate cyclase-independent mechanisms
.