Genetic analysis of the D1S80 locus in five North Indian populations

The distributions of the D1S80 alleles and genotypes in five endogamous pop ulations (Lobanas, Jat Sikhs, Brahmins, Khatris and Scheduled castes) was d etermined by amplified fragment length polymorphism (AMP-FLP) technique. Th e distributions of the observed genotypes for the five populations conforme d to Hardy-Weinberg expectations. Two alleles D1S80*18 and D1S80*24 were ob served in all populations at frequencies similar to many Caucasian populati ons, but they showed significant inter-population variability within the re gion. Allele *24 varied from 33% (Scheduled caste) to 49% (Lobanas), while the allele *18 frequency was lowest in Lobanas (15%) and highest in Jat Sik hs (25%). There was significant overall heterogeneity among the populations studied for this locus. The heterozygosity, probability of exclusion, matc h probability and discrimination probability estimates demonstrate the usef ulness of this locus for paternity and forensic purposes in Indian populati ons.