A novel and efficient regimen for producing chronic relapsing experimentalautoimmune encephalomyelitis (CR-EAE) in SJL mice

We report that SJL mice developed chronic relapsing experimental autoimmune encephalomyelitis (CR-EAE) when injected with a mixture of mouse spinal co rd homogenate (MSCH), killed mycobacteria tuberculosis (M. tb), and mycobac teria butyricum (M. b) in PBS 2 months before a conventional acute experime ntal autoimmune encephalomyelitis (EAE) induction injection. The altered pr ogression of the disease involved an accelerated but less severe acute atta ck and development of a chronic course with relapsing-remitting episodes. H istological examination revealed inflammatory cell infiltration and demyeli nation in the brain. The dose of neuroantigen as well as the anatomical sit es of injections were found to be crucial for the development of the diseas e.