In vitro analysis of the glucose-transport system in GLUT4-null skeletal muscle

We have characterized the glucose-transport system in soleus muscle from fe male GLUT4-null mice to determine whether GLUT1, 3 or 5 account for insulin -stimulated glucose-transport activity. Insulin increased 2-deoxyglucose up take 2.8- and 2.1-fold in soleus muscle from wild-type and GLUT4-null mice, respectively. Cytochalasin B, an inhibitor of GLUT1- and GLUT4-mediated gl ucose transport, inhibited insulin-stimulated 2-deoxyglucose uptake by > 95 % in wild-type and GLUT4-null soleus muscle. Addition of 35 mM fructose to the incubation media was without effect on insulin-stimulated 3-O-methyl-gl ucose transport activity in soleus muscle from either genotype, whereas 35 mM glucose inhibited insulin-stimulated (20 nM) 3-O-methylglucose transport by 65% in wild-type and 99% in GLUT4-null mice. We utilized the 2-N-4-1-(1 -azi-2,2,2-trifluoroethyl)benzoyl-1,3-bis(D-mannose-4-yloxy)-2-propylamine (ATB-BMPA) exofacial photolabel to determine if increased cell-surface GLUT 1 or GLUT4 content accounted for insulin-stimulated glucose transport in GL UT4-null muscle. In wild-type soleus muscle, cell-surface GLUT4 content was increased by 2.8-fold under insulin-stimulated conditions and this increas e corresponded to the increase in 2-deoxyglucose uptake. No detectable cell -surface GLUT4 was observed in soleus muscle from female GLUT4-null mice un der either basal or insulin-stimulated conditions. Basal cell-surface GLUT1 content was similar between wild-type and GLUT4-null mice, with no further increase noted in either genotype with insulin exposure. Neither GLUT3 nor GLUTS appeared to account for insulin-stimulated glucose-transport activit y in wild-type or GLUT4-null muscle. In conclusion, insulin-stimulated gluc ose-transport activity in female GLUT4-null soleus muscle is mediated by a facilitative transport process that is glucose- and cytochalasin B-inhibita ble, but which is not labelled strongly by ATB-BMPA.