V. Arluison et al., RNA : pseudouridine synthetase Pus1 from Saccharomyces cerevisiae: Oligomerization property and stoichiometry of the complex with yeast tRNA(Phe), BIOCHIMIE, 81(7), 1999, pp. 751-756
Yeast RNA:pseudouridine synthetase Pus1 catalyzes the formation of pseudour
idines in tRNAs. We report here the quaternary structure of purified recomb
inant Pus1 in solution. At low concentration, in the absence of tRNA, Pus1
oligomerizes while at high concentration it precipitates. This oligomerizat
ion/aggregation can be prevented by addition of dodecyl-beta-D-maltoside or
of yeast tRNA(Phe). The detergent does not significantly interfere with su
bstrate binding or with activity of Pus1. The stoichiometry of the Pus1/tRN
A(Phe) complex is 1/1. We conclude that the detergent covers an hydrophobic
region of the RNA binding pocket responsible for Pus1 aggregation. (C) 199
9 Societe francaise de biochimie et biologie moleculaire / Editions scienti
fiques et medicales Elsevier SAS.