Analysis of the disruption mutant of the oscillin homolog gene of Dictyostelium discoideum

Citation
Y. Matsuda et al., Analysis of the disruption mutant of the oscillin homolog gene of Dictyostelium discoideum, BIOL PHAR B, 22(9), 1999, pp. 915-919
Citations number
15
Categorie Soggetti
Pharmacology & Toxicology
Journal title
BIOLOGICAL & PHARMACEUTICAL BULLETIN
ISSN journal
09186158 → ACNP
Volume
22
Issue
9
Year of publication
1999
Pages
915 - 919
Database
ISI
SICI code
0918-6158(199909)22:9<915:AOTDMO>2.0.ZU;2-9
Abstract
A homolog of oscillin, the Ca2+ oscillation-inducing factor of the hamster, was identified from the cellular slime mold Dictyostelium discoideum and d esignated Dd-oscillin. In the developmental stages of D. discoideum, the ge ne is expressed at the prestalk region which contains a higher concentratio n of cytosolic Ca2+ than the prespore region. The Dd-oscillin null strain a ggregated but did not develop further when the cells were plated on non-nut rient agar at a density of 1.5x10(6) cells/cm(2), showing that the Dd-oscil lin gene is important for development. Since the null cells carrying the ha mster oscillin gene formed fruiting body, the hamster oscillin was the homo log of Dd-oscillin as far as function is concerned. In addition, the null c ells formed fruiting body in the presence of 2,5-di(tert-butyl)-1,4-hydroqu inone (BHQ: a specific inhibitor of Ca2+-ATPase activity in the endoplasmic reticulum). These results suggest that Dd-oscillin will increase cytosolic Ca2+ in the cells and promote further development.