Changes in lactate dehydrogenase and 3-hydroxyacetyl-CoA dehydrogenase activities in rat skeletal muscle by the administration of Eucommia ulmoides Oliver leaf with spontaneous running-training

We examined the effect of Eucommia ulmoides OLIVER leaf on rat skeletal mus cles together with spontaneous running-training in terms of the isozyme pro file and specific activity of lactate dehydrogenase (LDH; EC 1.1.1.27) and 3-hydroxyacetyl-CoA dehydrogenase (HAD; EG 1.1.1.35). On the twenty-ninth d ay of the experimental period, a mandatory endurance running exercise (trea dmill, 7 degrees grade) was conducted. Twenty-four hours later, the rats we re sacrificed and the skeletal muscles and other organs were dissected, Due to the training, the HAD specific activity in the skeletal muscles had inc reased and a more oxidative metabolism had developed, which was further enh anced by the administration of the leaf, In soleus (SOL) muscle in the Euco mmia leaf treated running-training group (ET), the LDH specific activity in the skeletal muscle was significantly higher than in the sedentary control group (SC), The isozyme profile of the group ET was significantly differen t when compared with the group SC. The changes in the LDH isozyme profile w ere larger in the SOL than that in extensor digitorum longus (EDL) muscle. The results show that mechanical training and the use of the leaf cooperati vely increase the ability to avoid lactate accumulation in skeletal muscle. This effect is supported by the group where 67% of rats accomplished the e ndurance running exercise, Theses results suggest that the administration o f Eucommia ulmoides OLIVER leaf along with light intensity training enhance s the ability of a muscle to resist fatigue.