L. Hunt et al., GFP-expressing mammalian cells for fast, sensitive, noninvasive cell growth assessment in a kinetic mode, BIOTECH BIO, 65(2), 1999, pp. 201-205
This study correlates the fluorescent signal from stable recombinant CHO ce
ll lines expressing the green fluorescent protein (GFP) at high levels with
biomass or cell number, extending the use of fluorescent proteins to appli
cations and assays where cell growth rates are important. Using a standard
fluorometer, growth of these cells can be quantified noninvasively in multi
well plates, and because signals are obtained without preparation, the same
culture samples can be measured repeatedly. Even with a small relative cha
nge in biomass, the specific growth rate can be determined in a few hours.
The dynamics of cell populations can now be studied with high sensitivity,
low error rate, and minimum sample preparation. (C) 1999 John Wiley & Sons,
Inc.