M. Abrahams et al., Effects of propofol on extracellular acidification rates in primary cortical cell cultures: application of silicon microphysiometry to anaesthesia, BR J ANAEST, 83(3), 1999, pp. 467-469
Citations number
8
Categorie Soggetti
Aneshtesia & Intensive Care","Medical Research Diagnosis & Treatment
Propofol depresses both cerebral oxygen consumption and glucose utilization
. We tested the hypothesis that these well described effects on brain metab
olism are manifest by a reduction in neuronal acid production in vitro. The
rate of extracellular acidification in primary cell cultures of rat cortic
al neurones was measured using a novel instrument (silicon microphysiometer
) after stimulation with propofol 0.3, 3 and 30 mu g ml(-1). Intralipid 10%
served as a control. Propofol 3 mu g ml(-1) caused a mean decrease of 1.51
(SEM 0.71)% in baseline acidification rate, which was significantly greate
r than that produced by 0.3 mu g ml(-1) or Intralipid alone (P<0.05). The r
eduction after stimulation with propofol 30 mu g ml(-1) was 4.68 (0.35)% of
baseline rates and this in turn was significantly greater than that elicit
ed by propofol 3 or 0.3 mu g ml(-1), or Intralipid (P<0.001). We have confi
rmed the depressant effect of propofol on cerebral metabolism and establish
ed that propofol inhibits neuronal acid excretion in vitro.