Involvement of PI 3-kinase and activated ERK in facilitating insulin-stimulated triacylglycerol synthesis in hepatocytes

Triacylglycerol synthesis was studied in hepatocytes isolated from fasted/r efed rats by EDTA perfusion. Insulin induced a 1.5-fold increase in glucose incorporation into triacylglycerol. Insulin-stimulated triacylglycerol syn thesis and insulin-stimulated protein kinase B/Akt activity were inhibited by the phosphatidylinositol 3-kinase inhibitors wortmannin and LY 294002, a nd the mitogen-activated protein kinase kinase inhibitor PD 98059. Inhibiti on of p70 ribosomal protein-Sb kinase with rapamycin was without effect. In sulin-stimulated pyruvate dehydrogenase nase activity was abolished by phos phatidylinositol 3-kinase inhibitors. No effect of insulin on acetyl CoA ca rboxylase activity was observed. CELL SIGNAL 11;10:713-717, 1999. (C) 1999 Elsevier Science Inc.