Hybridization-based karyotyping of mouse chromosomes: hybridization-bands

We have developed a method, which we have named hybridization-banding, to i dentify simultaneously all chromosomes in a mouse metaphase spread. The met hod uses a combination of hybridization probes labeled with a single fluor to yield a simple, unique, readily identifiable hybridization pattern on ea ch chromosome. The method is superior to Giemsa- or fluorescence-based band ing methods for chromosome identification because the hybridization pattern s are simpler and easier to identify, and unique patterns can be designed a t will for each chromosome. Analysis can be performed with a standard fluor escence microscope, and images call be recorded on film with an ordinary 35 -mm camera, making the method useful to many investigators. The method can also be applied to any species for which chromosomes and probes can be prep ared.