Xj. Zhang et al., Glucose nanosensor based on Prussian-blue modified carbon-fiber cone nanoelectrode and an integrated reference electrode, ELECTROANAL, 11(13), 1999, pp. 945-949
An enzyme nanosensor, based on a carbon fiber cone nanoelectrode modified b
y codeposition of Prussian blue (PB) and glucose oxidase (GOD), has been co
nstructed. The nanosensor displays a low-potential electrocatalytic detecti
on of the enzymatically liberated hydrogen peroxide, along with good reprod
ucibility and high selectivity. The dependence of the nanosensor response u
pon parameters of the sensor preparation (e.g., amount of GOD, PB depositio
n time, Nafion coverage), and upon measurements conditions (e.g., pH, appli
ed potential) was evaluated. An operating potential of -0.1 V (vs. Ag/AgCl)
yielded the highest selectivity towards glucose, with no interference from
ascorbic acid. The lifetime of the nanosensor is more than two weeks. Such
miniaturization and analytical performance offer great promise for measure
ments of glucose in extremely small volumes.