Molecular cloning of mXCR1, the murine SCM-1/lymphotactin receptor

Single C motif-1 (SCM-1)/lymphotactin is a C-type member of the chemokine s uperfamily, Previously, we identified its specific receptor XCR1. Here we i solated the murine homologue of XCR1 (mXCR1). To demonstrate its biological activity, we produced recombinant mouse SCM-1 by the baculovirus expressio n system, B300-19 murine pre-B cells expressing mXCR1 responded to mSCM-1 i n chemotactic and calcium-mobilization assays. mXCR1 mRNA was weakly expres sed in spleen and lung of normal C57BL/6 mice. In spleen, CD8(+) cells and NK1.1(+) cells were found to express mXCR1. Identification of mXCR1 will no w allow us to study the role of this unique cytokine system in the mouse mo dels of inflammation and immunity, (C) 1999 Federation of European Biochemi cal Societies.