Scanning electrochemical microscopy of enzymes immobilized on structured glass-gold substrates

Scanning electrochemical microscopy (SECM) was used to characterize enzyme- modified glass-gold specimens. The exposed gold surface was functionalized with an aminothiol and reacted with carbodiimide-activated glucose oxidase. The specimen surface was examined with SECM, using a 25 mu m platinum elec trode. Images were acquired showing the topography, electric conductivity, and enzymatic activity of the composite surface. It was found that the hydr oxy-groups of the glass surface are as likely to bind to the activated enzy me as the amino-groups on the gold surface.