Enzymatic synthesis of nucleotide sugars

The present review gives a survey on the biosynthetic pathways of nucleotid e sugars which are important for the in vitro synthesis of mammalian glycoc onjugates. With respect to the use of these enzymes in glycotechnology the availability as recombinant enzymes from different sources, the large-scale synthesis of nucleotide sugars and their in situ regeneration in combinati on with glycosyltransferases are summarized and evaluated.