ENHANCEMENT OF HUMORAL IMMUNITY TO SIVENV FOLLOWING SIMULTANEOUS INOCULATION OF MICE BY 3 RECOMBINANT ADENOVIRUSES ENCODING SIVENV POLIOVIRUS CHIMERAS, TAT AND REV/

A means of inducing gene expression by simultaneous infection with thr ee recombinant adenoviruses (Ad) is described, The simian immunodefici ency virus (SIV) envelope-coding region was placed under the control o f the human immunodeficiency virus type 1 (HIV-1) Tat and Rev proteins provided in trans by distinct Ad vectors (Ad-tat; Ad-rev), Coinfectio n of cells with the three recombinant adenoviruses led to induction of high levels of SIV env mRNA and protein synthesis, while inoculation of mice elicited anti-Env antibodies, Insertion of the poliovirus VP1 neutralization epitope (C3) in the V1 hypervariable region of SIV enve lope not only proved to be highly immunogenic for the poliovirus epito pe but also enhanced the kinetics of anti-SIV Env antibody production, By contrast, insertion in V4 elicited no anti-C3 response and only no rmal anti-Env responses.