The effect of microhabitat on the chironomid (Diptera) community of a large
river was tested by trapping floating pupal exuviae after emergence of adu
lts. The spatial distribution of the 33 species or groups of species that w
ere identified was strongly explained by mean particle size, porosity and h
eterogeneity of substratum. A study of the size, means of respiration, mode
of life and feeding habits of the species showed that the substratum-fauna
l relationships could be explained in part by biological traits of the spec
ies present. Two gradients could be defined on the basis of habitat variabl
es: a substratum porosity gradient that was significantly correlated with s
pecies richness and diversity, and a second gradient related to particle si
ze that was significantly correlated with biological categories.