Sequence analysis and expression of a mRNA for a larval-specific cuticularprotein, LCP1, from Helicoverpa armigera

Several cDNA clones for a larval cuticular protein from Helicoverpa armiger a were isolated and sequenced. The cDNA clones contain an open reading fram e encoding a 109 residue protein which is homologous to other known cuticul ar proteins. The predicted protein appears to have a signal peptide which w ould be removed to give a mature protein of 91 amino acid residues with an M-r of 10 127. The mature protein, LCP1 (Larval Cuticular Protein), would b e highly acidic, as is found for other cuticular proteins from flexible ins ect cuticles. The mRNA appears to be expressed throughout larval developmen t although it is more highly expressed in the integument of the late final instar.